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Tips and tricks on how to perform hybridization step of FISH

The FISH protocol consists of 3 main procedures: cells/tissue preparation, probe hybridization, and washing (post-hybridization).

It is critical for the hybridization step to have all optimal conditions to have a successful in situ result, including elevated temperature and elongated time of the hybridization reaction (usually overnight).

These two factors increase the possibility of buffer evaporation during the process, leading to drying out of the sample.

We prepared for you a tutorial video on how to increase experiment performance and avoid the loss of your sample during hybridization process.


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